Monkey Granzyme A ELISA Kit (A327082)

Monkey Granzyme A ELISA Kit

Monkey Granzyme A ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay designed for the in vitro quantitative determination of monkey Granzyme A in serum, plasma, and other biological fluids.

Sandwich (quantitative)

Monkey Granzyme A ELISA Kit (A327082) employs the sandwich enzyme immunoassay technique for the quantitative measurement of monkey Granzyme A in serum, plasma, and other biological fluids. An antibody specific for Granzyme A has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Granzyme A present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Granzyme A Antibody, which binds the captured Granzyme A present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Granzyme A captured in each well. The concentration of Granzyme A can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colormetric

Colorimetric Microplate Reader

Serum, plasma, and other biological fluids.

15.63-1000 pg/ml

3h 30m

Monkey

No significant cross-reactivity or interference between monkey Granzyme A and analogues was observed.

Intra-assay CV: 5.33%, Inter-assay CV: 5.13%

Shipped at +4°C. Store short term at +4°C and long term at +4°C/-20°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.

CTL tryptase, CTLA3, Cytotoxic T-lymphocyte proteinase 1, Fragmentin-1, Granzyme-1, GZMA, H factor, Hanukkah factor, HF, HFSP

Information online is representative. Always refer to the Product Manual inside the kit.