Monkey IgA ELISA Kit (A75128)

Monkey IgA ELISA Kit

Monkey IgA ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of monkey IgA in serum, plasma, tissue homogenates, and other biological fluids.

Sandwich (quantitative)

Monkey IgA ELISA Kit (A75128) employs HRP antibody sandwich ELISA for the quantitative measurement of monkey IgA in serum, plasma, tissue homogenates, and other biological fluids. Capture antibody specific for IgA is pre-coated on a 96-well microtiter plate. Samples and standards are added, and IgA binds to the immobilized antibody. Detection is performed using HRP-conjugated detection antibody, which binds to a different epitope on the captured target. Following another wash, TMB substrate solution produces a blue-coloured product that changes to yellow after addition of acidic stop solution. The intensity of yellow is proportional to the amount of IgA captured, and the concentration is determined by reading O.D. at 450nm.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric

Colorimetric Microplate Reader

1.875 ng/ml

3.125-200 ng/ml

4 hours

Monkey

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Store at +4°C. Do not use past expiration date!

Ig alpha-1 chain C region, Ig alpha-1 chain C region BUR, Ig alpha-1 chain C region TRO, Ig alpha-2 chain C region, Ig alpha-2 chain C region BUT, Ig alpha-2 chain C region LAN, IGHA1, IGHA2, Immunoglobulin heavy constant alpha 1, Immunoglobulin heavy constant alpha 2

Information online is representative. Always refer to the Product Manual inside the kit.