Monkey IgM ELISA Kit (A75516)

Monkey IgM ELISA Kit

Monkey IgM ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of monkey IgM in serum, plasma, tissue homogenates, and other biological fluids.

Sandwich (quantitative)

Monkey IgM ELISA Kit (A75516) employs HRP antibody sandwich ELISA for the quantitative measurement of monkey IgM in serum, plasma, tissue homogenates, and other biological fluids. Capture antibody specific for IgM is pre-coated on a 96-well microtiter plate. Samples and standards are added, and IgM binds to the immobilized antibody. Detection is performed using HRP-conjugated detection antibody, which binds to a different epitope on the captured target. Following another wash, TMB substrate solution produces a blue-coloured product that changes to yellow after addition of acidic stop solution. The intensity of yellow is proportional to the amount of IgM captured, and the concentration is determined by reading O.D. at 450nm.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric

Serum, plasma, tissue homogenates, and other biological fluids.

0.938 ng/ml

1.563-100 ng/ml

3 hours

Monkey

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Store at +4°C. Do not use past expiration date!

Ig mu chain C region, Ig mu chain C region BOT, Ig mu chain C region GAL, Ig mu chain C region OU, IGHM, Immunoglobulin heavy constant mu

Information online is representative. Always refer to the Product Manual inside the kit.