Mouse Adiponectin ELISA Kit (A1548)

Mouse Adiponectin ELISA Kit

Mouse Adiponectin ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Adiponectin in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Mouse Adiponectin ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse Adiponectin in the samples. A monoclonal antibody specific for mouse Adiponectin has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for Adiponectin, and Adiponectin present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Adiponectin bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

0.73 pg/ml

31.25 - 2000 pg/ml

This kit can assay both native and recombinant mouse Adiponectin.

Mouse

This ELISA kit has shown no significant cross-reactivity.

Store at +4°C. Do not use past expiration date!

30 kDa adipocyte complement-related protein, ACDC, ACRP30, Adipocyte complement-related 30 kDa protein, Adipocyte, C1q and collagen domain-containing protein, ADIPOQ, Adipose most abundant gene transcript 1 protein, apM-1, APM1, GBP28, Gelatin-binding protein

Information online is representative. Always refer to the Product Manual inside the kit.