Mouse CCL20 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse CCL20 in the samples. A monoclonal antibody specific for mouse CCL20 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for CCL20, and CCL20 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of CCL20 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Cell culture supernatant, serum and plasma.
Sensitivity
5.82 pg/ml
Product Range
31.25 - 2000 pg/ml
Specificity
This kit can assay both natural and recombinant mouse CCL20.
Reactivity
Mouse
Cross Reactivity
This assay has shown no significant cross-reactivity.
Recovery
90% (Average Recovery from Serum Samples).
Storage
Store at 2 - 8°C.
Synonyms
Beta-chemokine exodus-1, C-C motif chemokine 20, CC chemokine LARC, LARC, Liver and activation-regulated chemokine, Macrophage inflammatory protein 3 alpha, MIP-3-alpha, MIP3A, SCYA20, Small-inducible cytokine A20
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
