Mouse CCL22 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse CCL22 in cell culture supernatant, serum and plasma.
Assay Type
Sandwich (quantitative)
Principle of Assay
Mouse CCL22 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse CCL22 in the samples. A monoclonal antibody specific for mouse CCL22 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for CCL22, and CCL22 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of CCL22 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Cell culture supernatant, serum and plasma.
Sensitivity
4.07 pg/ml
Product Range
31.25 - 2000 pg/ml
Specificity
This kit can assay both native and recombinant mouse CCL22.
Reactivity
Mouse
Cross Reactivity
This ELISA kit has shown no significant cross-reactivity.
Recovery
84% (Average Recovery from Serum Samples).
Storage
Store at +4°C. Do not use past expiration date!
Synonyms
C-C motif chemokine 22, CC chemokine STCP-1, Macrophage-derived chemokine, MDC(1-69, SCYA22, Small-inducible cytokine A22, Stimulated T-cell chemotactic protein 1
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
