Mouse CCL4 ELISA Kit (A1555)

Mouse CCL4 ELISA Kit

Mouse CCL4 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse CCL4 in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Mouse CCL4 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse CCL4 in the samples. A monoclonal antibody specific for mouse CCL4 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for CCL4, and CCL4 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of CCL4 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

2.12 pg/ml

31.25 - 2000 pg/ml

This kit can assay both native and recombinant mouse CCL4.

Mouse

This ELISA kit has shown no significant cross-reactivity.

88% (Average Recovery from Serum Samples).

Store at +4°C. Do not use past expiration date!

C-C motif chemokine 4, G-26 T-lymphocyte-secreted protein, HC21, LAG-1, LAG1, Lymphocyte activation gene 1 protein, Macrophage inflammatory protein 1-beta, MIP-1-beta, MIP-1-beta(1-69, MIP1B, SCYA4

Information online is representative. Always refer to the Product Manual inside the kit.