Mouse CXCL1 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse CXCL1 in cell culture supernatant, serum and plasma.
Assay Type
Sandwich (quantitative)
Principle of Assay
Mouse CXCL1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse CXCL1 in the samples. A monoclonal antibody specific for mouse CXCL1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, and CXCL1 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked monoclonal antibody specific for CXCL1 is added to the wells. Following a wash to remove any unbound detect antibody, streptavidin-HRP is added. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of CXCL1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Cell culture supernatant, serum and plasma.
Sensitivity
0.47 pg/ml
Product Range
15.63 - 1000 pg/ml
Specificity
This kit can assay both native and recombinant mouse CXCL1.
Reactivity
Mouse
Cross Reactivity
This ELISA kit has shown no significant cross-reactivity.