Mouse FVIII ELISA Kit (A75422)

Mouse FVIII ELISA Kit

Sandwich (quantitative)

This Mouse FVIII ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse FVIII in plasma, tissue homogenates and other biological fluids.

An antibody specific for FVIII has been coated on a microplate. Standards and samples are pipetted into the wells and the FVIII present in each sample is bound to the wells by the immobilised antibody. The wells are washed and Anti-FVIII Antibody (Biotin) is added. After washing away the unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are washed again, before a TMB substrate solution is added to the wells and colour develops in proportion to the amount of FVIII bound. The Stop Solution changes the colour from blue to yellow, and the intensity of the colour is measured at 450 nm.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric

Colorimetric Microplate Reader

Serum, plasma, tissue homogenates, and other biological fluids.

0.156-10 ng/ml

4h 30m

Mouse

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Information online is representative. Refer to the Instructions for Use inside the kit.

Store at +4°C. Do not use past expiration date!

AHF, Antihemophilic factor, Coagulation factor VIII, F8, F8C, Procoagulant component

Information online is representative. Always refer to the Product Manual inside the kit.