Mouse ICAM1 ELISA Kit (A1601)

Mouse ICAM1 ELISA Kit

Mouse ICAM1 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse ICAM1 in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Mouse sICAM-1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse sICAM-1 in the samples. A monoclonal antibody specific for mouse sICAM-1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for sICAM-1, and sICAM-1 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of sICAM-1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

1.27 pg/ml

125 - 8000 pg/ml

This kit can assay both native and recombinant mouse sICAM-1.

Mouse

This ELISA kit has shown no significant cross-reactivity.

102% (Average Recovery from Serum Samples).

Store at +4°C. Do not use past expiration date!

ICAM-1, Intercellular adhesion molecule 1, Major group rhinovirus receptor

Information online is representative. Always refer to the Product Manual inside the kit.