Mouse IFN gamma ELISpot Kit (A334660)

Mouse IFN gamma ELISpot Kit

Mouse IFN gamma ELISpot Kit is a ready-to-use ELISpot assay for the in vitro detection and quantification of IFN gamma-secreting mouse splenocytes at the single-cell level.

sandwich ELISA

The Mouse IFN gamma ELISpot Kit (A334660/A334661) employs the double-antibody sandwich ELISpot technique for the detection of IFN gamma-secreting cells from mouse lymphocytes. Sample lymphocytes are isolated from mouse spleen tissue after mechanical grinding and added to a 96-well microplate (A334660) or detachable strip (A334661) pre-coated with anti-IFN gamma capture antibody. Upon stimulation with a cell stimulant, IFN gamma secreted by individual cells is captured by the immobilized antibody. A biotinylated anti-IFN gamma detection antibody, which also binds the captured cytokine, and Streptavidin-HRP, which binds the biotinylated detection antibody, are then added, and the plate is incubated. Following incubation, unbound detection antibody and Streptavidin-HRP are removed by washing, and AEC substrate solution is added. Colored spots develop at the sites of cytokine secretion, which can be quantified using an ELISpot reader. This ready-to-use kit includes pre-coated plates/strips, detection antibody, Streptavidin-HRP, AEC coloring system, dilution buffer, cell stimulant, and washing buffer.

Biotinylated Antibody 100µl 1 vial; Streptavidin-HRP 100µl 1 vial; Cell Stimulant 50 T 1 bottle; Dilution Buffer R (1×) 10mL 2 bottles; Washing Buffer (50×) 15mL 1 bottle; AEC Dilution 10mL 1 bottle; AEC Solution I (20×) 500µl 1 vial; AEC Solution II (20×) 500µl 1 vial; AEC Solution III (200×) 50µl 1 vial; Pre-coated PVDF Plate/1 plate.

Colorimetric

ELISpot Plate Reader or Image Capture and Quantification. Spots on the membrane are counted, with each spot corresponding to one secreting cell, providing a quantitative measure of cellular activity.

Peripheral blood mononuclear cells (PBMCs) in single-cell suspension, capable of cytokine secretion.

Assay time: 3 hours. Sample incubation time: 16-24 hours.

This ELISpot Kit specifically targets Mouse IFN gamma.

Mouse

Intra-assay CV: < 15%, Inter-assay CV: < 20%

Shipped at 4°C. The kit should be stored at 2-8°C. Shelf life is 12 months from the date of receipt.

IFN-gamma, IFNG, Immune interferon, Interferon gamma

Information online is representative. Always refer to the Product Manual inside the kit.