Mouse IgA ELISA Kit (High Range) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of high-abundance mouse IgA in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.
Assay Type
Sandwich (quantitative)
Principle of Assay
Mouse IgA ELISA Kit (High Range) (A78290) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse IgA in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for IgA. Standards and samples are added to the wells, where IgA binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and an HRP-conjugated detection antibody specific for IgA is added to the wells. The detection antibody binds the captured IgA to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of IgA captured in each well. The concentration of IgA is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
Platform
Pre-coated Microplate (12 x 8 well strips)
Detection Type
Colorimetric Detection (OD450)
Instrument
Colorimetric Microplate Reader
Sample Type
Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.
Sensitivity
0.938 ng/mL
Product Range
1.563-100 ng/mL
Assay Time
4 hours
Reactivity
Mouse
Cross Reactivity
Specific for IgA with no reported cross-reactivity with other analogs or variants.
Recovery
Sample Type
n
Range
Average
Serum
10
90% - 104%
97%
EDTA Plasma
10
87% - 102%
96%
Heparin Plasma
10
87% - 103%
96%
Linearity
Sample Type
n
1:2
1:4
1:8
Serum
10
91-101%
82-97%
92-99%
EDTA Plasma
10
89-99%
88-91%
83-98%
Heparin Plasma
10
86-98%
86-95%
80-96%
Storage
Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.
Synonyms
Ig alpha-1 chain C region, Ig alpha-1 chain C region BUR, Ig alpha-1 chain C region TRO, Ig alpha-2 chain C region, Ig alpha-2 chain C region BUT, Ig alpha-2 chain C region LAN, IGHA1, IGHA2, Immunoglobulin heavy constant alpha 1, Immunoglobulin heavy constant alpha 2
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Linearity assessment of the Mouse IgA ELISA Kit (A78290) across serial sample dilutions. Linearity was evaluated using Serum, EDTA Plasma, and Heparin Plasma samples serially diluted from 1:2 to 1:8. Recovery (%) was calculated relative to the expected Mouse IgA concentration at each dilution. Data are presented as mean recovery values from 10 replicates (n = 10).
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