Principle of Assay
Mouse IgE ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse IgE in the samples. A monoclonal antibody specific for mouse IgE has been immobilized onto microwells. Standard or samples are pipetted into the wells, and IgE present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for IgE is added to the wells. Following a wash to remove any unbound detect antibody, streptavidin-HRP is added. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IgE bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
