Mouse IgG ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse IgG in cell culture supernatant, serum and plasma.
Assay Type
Sandwich (quantitative)
Principle of Assay
Mouse IgG ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse IgG in the samples. A monoclonal antibody specific for mouse IgG has been immobilized onto microwells. Standard, samples and HRP-linked detect antibody specific for IgG are pipetted into the wells and IgG present is bound by the immobilized antibody. After washing away any unbound substances, substrate solution is added to the wells and color develops in proportion to the amount of IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Cell culture supernatant, serum and plasma.
Sensitivity
14.70 pg/ml
Product Range
3.13 - 200 ng/ml
Reactivity
Mouse
Storage
Store at +4°C. Do not use past expiration date!
Synonyms
Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1, Immunoglobulin heavy constant gamma 1
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
