Mouse IL-12 p40 ELISA Kit (A1575)

Mouse IL-12 p40 ELISA Kit

Mouse IL-12 p40 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse IL-12 p40 in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Mouse IL-12 p40 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse IL-12 p40 in the samples. A monoclonal antibody specific for mouse IL-12 p40 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for IL-12 p40, and IL-12 p40 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-12 p40 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

0.25 pg/ml

4.69 - 300 pg/ml

This kit can assay both native and recombinant mouse IL-12 p40.

Mouse

This ELISA kit has shown no significant cross-reactivity.

98% (Average Recovery from Serum Samples).

Store at +4°C. Do not use past expiration date!

CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, IL-12B, IL12B, Interleukin-12 subunit beta, NK cell stimulatory factor chain 2, NKSF2

Information online is representative. Always refer to the Product Manual inside the kit.