Mouse IL-17E ELISA Kit (A1580)

Mouse IL-17E ELISA Kit

Mouse IL-17E ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse IL-17E in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Mouse IL-17E ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse IL-17E in the samples. A monoclonal antibody specific for mouse IL-17E has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for IL-17E, and IL-17E present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-17E bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

1.22 pg/ml

62.50 - 4000 pg/ml

This kit can assay both native and recombinant mouse IL-17E.

Mouse

This ELISA kit has shown no significant cross-reactivity.

83% (Average Recovery from Serum Samples).

Store at +4°C. Do not use past expiration date!

IL17E, IL25, Interleukin-17E, Interleukin-25

Information online is representative. Always refer to the Product Manual inside the kit.