Mouse IL-17F ELISA Kit (A1581)

Mouse IL-17F ELISA Kit

Mouse IL-17F ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse IL-17F in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Mouse IL-17F ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse IL-17F in the samples. A monoclonal antibody specific for mouse IL-17F has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for IL-17F, and IL-17F present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-17F bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

0.59 pg/ml

31.25 - 2000 pg/ml

This kit can assay both native and recombinant mouse IL-17F.

Mouse

This ELISA kit has shown no significant cross-reactivity.

106% (Average Recovery from Serum Samples).

Store at +4°C. Do not use past expiration date!

Cytokine ML-1, IL17F, Interleukin-17F

Information online is representative. Always refer to the Product Manual inside the kit.