Principle of Assay
Mouse IL-27 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse IL-27 in the samples. A monoclonal antibody specific for mouse IL-27 has been immobilized onto microwells. Standard or samples are pipetted into the wells, and IL-27 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked monoclonal antibody specific for IL-27 is added to the wells. Following a wash to remove any unbound detect antibody, streptavidin-HRP is added. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-27 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
