Mouse IL23 ELISA Kit (A634)

Mouse IL23 ELISA Kit

Sandwich (quantitative)

This IL23 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-IL23 Antibody. Standards or samples are then added to the microtire plate wells and IL23, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of IL23 present in the sample, Anti-IL23 Antibody (Biotin) is added to each well to "sandwich" the IL23 immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL23 will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Microplate

Colorimetric

< 2 pg/ml

31.2-2000 pg/ml

Mouse

Store at 2-8°C for 6 months or at -20°C for 12 months.

IL 23, IL 23 A, IL 23 subunit alpha, IL 23A, IL 23p19, IL-23 subunit alpha, IL-23-A, IL-23p19, IL12B, Il23a, IL23A_HUMAN, IL23P19, interleukin 12B, Interleukin 23 alpha subunit p19, Interleukin 23 p19 subunit, interleukin 23 subunit alpha, interleukin 23 subunit p19, interleukin six, G CSF related factor, Interleukin-23 subunit alpha, Interleukin-23 subunit p19, JKA3 induced upon T cell activation, MGC79388, P19, SGRF

Information online is representative. Always refer to the Product Manual inside the kit.