Mouse M-CSF ELISA Kit (A1597)

Mouse M-CSF ELISA Kit

Mouse M-CSF ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse M-CSF in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Mouse M-CSF ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse M-CSF in the samples. A monoclonal antibody specific for mouse M-CSF has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for M-CSF, and M-CSF present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of M-CSF bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

6.06 pg/ml

31.25 - 2000 pg/ml

This kit can assay both native and recombinant mouse M-CSF.

Mouse

This ELISA kit has shown no significant cross-reactivity.

92% (Average Recovery from Serum Samples).

Store at +4°C. Do not use past expiration date!

CSF-1, CSF1, Lanimostim, Macrophage colony-stimulating factor 1, MCSF, PG-M-CSF, Proteoglycan macrophage colony-stimulating factor

Information online is representative. Always refer to the Product Manual inside the kit.