Mouse Osteopontin ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Osteopontin in cell culture supernatant, serum and plasma.
Assay Type
Sandwich (quantitative)
Principle of Assay
Mouse Osteopontin ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse OPN in the samples. A monoclonal antibody specific for mouse OPN has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for OPN, and OPN present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of OPN bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Cell culture supernatant, serum and plasma.
Sensitivity
0.05 pg/ml
Product Range
15.63 - 1000 pg/ml
Specificity
This kit can assay both native and recombinant mouse OPN.
Reactivity
Mouse
Cross Reactivity
This ELISA kit has shown no significant cross-reactivity.
Storage
Store at +4°C. Do not use past expiration date!
Synonyms
BNSP, Bone sialoprotein 1, Nephropontin, OPN, Secreted phosphoprotein 1, SPP-1, SPP1, Urinary stone protein, Uropontin
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
