Mouse RANTES ELISA Kit (Small Sample Volume) is a 3h 30m sandwich Enzyme-Linked Immunosorbent Assay (sELISA) optimized for small sample volumes requiring only 25 µL of sample, designed for the in vitro quantitative determination of mouse RANTES in serum, plasma, and other biological fluids.
Assay Type
Sandwich (quantitative)
Principle of Assay
Mouse RANTES ELISA Kit (Small Sample Volume) (A323214) employs the sandwich Enzyme-Linked Immunoassay technique for the quantitative measurement of mouse in serum, plasma, and other biological fluids. This assay is optimized for limited sample availability, requiring only 25 µL of sample per well. An antibody specific for RANTES has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the RANTES present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with biotinylated anti-RANTES antibody, which binds the captured RANTES present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of RANTES captured in each well. The concentration of RANTES can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
Platform
Pre-coated Microplate (12 x 8 well strips)
Detection Type
Colorimetric Detection (OD450)
Instrument
Colorimetric Microplate Reader
Sample Type
Serum, plasma, and other biological fluids.
Sensitivity
18.75 pg/mL
Product Range
31.25-2000 pg/mL
Assay Time
3h 30m
Reactivity
Mouse
Cross Reactivity
Specific for RANTES with no reported cross-reactivity with other analogs or variants.
Recovery
Sample Type
n
Range
Average
Serum
8
93 - 109%
101%
EDTA Plasma
8
95 - 109%
101%
Cell Culture Media
8
90 - 103%
97%
Storage
Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.
Synonyms
C-C motif chemokine 5, CCL5, D17S136E, EoCP, Eosinophil chemotactic cytokine, SCYA5, SIS-delta, Small-inducible cytokine A5, T cell-specific protein P228, T-cell-specific protein RANTES, TCP228
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Linearity assessment of the Mouse RANTES ELISA Kit (Small Sample Volume) (A323214) across serial sample dilutions. Linearity was evaluated using Serum, EDTA Plasma, and Cell Culture Media samples serially diluted from 1:2 to 1:16. Recovery (%) was calculated relative to the expected Mouse RANTES concentration at each dilution. Data are presented as mean recovery values from 8 replicates (n = 8).
Quick workflow summary for Small Sample Volume ELISA Kits. Samples or standards (25 µL per well) are incubated in antibody pre-coated microplate wells, allowing target analytes to bind the immobilized capture antibody. Following incubation, a biotinylated detection antibody and HRP conjugate are sequentially added with intermediate wash steps to form a sandwich immune complex. TMB substrate is then added for color development, and the enzymatic reaction is stopped prior to optical density measurement at 450 nm. This workflow is optimized for low input and limited-volume biological samples.
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