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Mouse/Rat Insulin like Growth Factor 1 ELISA Kit (A1609)

This product is discontinued

Mouse/Rat Insulin like Growth Factor 1 ELISA Kit (A1609) has been discontinued and is no longer available.

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Name
Mouse/Rat Insulin like Growth Factor 1 ELISA Kit
Description
Mouse/Rat Insulin like Growth Factor 1 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse, rat Insulin like Growth Factor 1 in cell culture supernatant, serum and plasma.
Assay Type
Sandwich (quantitative)
Principle of Assay
Mouse/Rat IGF-1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse/rat IGF-1 in the samples. A monoclonal antibody specific for mouse/rat IGF-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and IGF-1 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for IGF-1 is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of IGF-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Cell culture supernatant, serum and plasma.
Sensitivity
0.82 pg/ml
Product Range
15.63 - 1000 pg/ml
Specificity
This kit can assay both native and recombinant mouse/rat IGF-1.
Reactivity
Mouse, Rat
Cross Reactivity
This ELISA kit has shown no significant cross-reactivity.
Storage
Store at +4°C. Do not use past expiration date!
Synonyms
IBP1, IGF I, IGF IA, IGF IB, IGF-I, IGF1, IGF1A, IGF1_HUMAN, IGFI, IGFIA, Insulin like growth factor 1 (somatomedin C) , Insulin like growth factor IA, Insulin like growth factor IB, Insulin-like growth factor I, Mechano growth factor, MGF, OTTHUMP00000195080, OTTHUMP00000195081, OTTHUMP00000195082, OTTHUMP00000195083, OTTHUMP00000195084, Somatomedia C, Somatomedin C, Somatomedin-C
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.