Mouse Anti-SARS-CoV-2 (S-RBD) (delta B.1.617.2) IgA ELISA Kit (A319435)

Mouse Anti-SARS-CoV-2 (S-RBD) (delta B.1.617.2) IgA ELISA Kit

Mouse Anti-SARS-CoV-2 (S-RBD) (delta B.1.617.2) IgA ELISA Kit is a 90 minute indirect Enzyme-Linked Immunosorbent Assay (iELISA) designed for the in vitro quantitative determination of mouse Anti-SARS-CoV-2 (S-RBD) (delta B.1.617.2) IgA in serum, plasma, tissue homogenates, and other biological fluids.

Indirect (quantitative)

Mouse Anti-SARS-CoV-2 (S-RBD) (delta B.1.617.2) IgA ELISA Kit (A319435) is a 1h 30m indirect Enzyme-Linked Immunosorbent Assay (iELISA) designed for the in vitro quantitative determination of mouse Anti-SARS-CoV-2 (S-RBD) (delta B.1.617.2) IgA in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with assay-specific antigen. Standards and samples are added to the wells, where Anti-SARS-CoV-2 (S-RBD) (delta B.1.617.2) IgA present in the samples binds to the immobilized antigen. Following incubation, unbound components are removed by washing and an HRP-conjugated detection antibody is added to the wells. This detection antibody binds the captured Anti-SARS-CoV-2 (S-RBD) (delta B.1.617.2) IgA to form an indirect immune complex. After further incubation and washing, TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Anti-SARS-CoV-2 (S-RBD) (delta B.1.617.2) IgA captured in each well. The concentration of Anti-SARS-CoV-2 (S-RBD) (delta B.1.617.2) IgA is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric Detection (OD450)

Colorimetric Microplate Reader

0.094 ng/mL

0.156-10 ng/mL

1h 30m

Mouse

Specific for Anti-SARS-CoV-2 (S-RBD) (delta B.1.617.2) IgA with no reported cross-reactivity with other analogs or variants.

Information online is representative. Always refer to the Product Manual inside the kit.

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.

Information online is representative. Always refer to the Product Manual inside the kit.

This product is for research use only. It is not intended for diagnostic or therapeutic use.