Principle of Assay
Mouse Serum ELISA Kit (A326883) employs an enzyme immunoassay for the qualitative detection of mouse Serum in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The 96-well microtiter plate is coated with capture antibodies or antigens specific for Serum. Samples are added and incubated, followed by washing and addition of enzyme-conjugated detection molecules. TMB substrate is added to produce a blue-coloured product that turns yellow after acidic stop solution. The presence or absence of yellow color indicates the presence or absence of Serum in the sample.