Principle of Assay
Mouse sheep red blood cell Immunoglobulin M ELISA Kit (A335214) is based on Capture ELISA technology for the quantitative measurement of mouse sheep red blood cell Immunoglobulin M in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. An antibody specific for sheep red blood cell Immunoglobulin M has been pre-coated onto a 96-well microtiter plate. Samples and standards are added to the plate and incubated. After washing with wash buffer, the appropriate detection reagent (e.g., HRP-conjugated antigen or streptavidin) is added and incubated. Following another wash step, TMB substrate solution is used to visualize the enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after adding acidic stop solution. The density of yellow is proportional to the amount of sheep red blood cell Immunoglobulin M that has been captured on the plate. The concentration of sheep red blood cell Immunoglobulin M can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
