Mouse TNF-Related Activation-induced Cytokine ELISA Kit (A1605)

Mouse TNF-Related Activation-induced Cytokine ELISA Kit

Mouse TNF-Related Activation-induced Cytokine ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse TNF-Related Activation-induced Cytokine in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Mouse TRANCE ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse TRANCE in the samples. A monoclonal antibody specific for mouse TRANCE has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for TRANCE, and TRANCE present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of TRANCE bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

4.23 pg/ml

31.25 - 2000 pg/ml

This kit can assay both native and recombinant mouse TRANCE.

Mouse

This ELISA kit has shown no significant cross-reactivity.

100% (Average Recovery from Serum Samples).

Store at +4°C. Do not use past expiration date!

CD254, ODF, OPGL, Osteoclast differentiation factor, Osteoprotegerin ligand, Receptor activator of nuclear factor kappa-B ligand, TNFSF11, TRANCE, Tumor necrosis factor ligand superfamily member 11

Information online is representative. Always refer to the Product Manual inside the kit.