Mouse Toxoplasma IgM ELISA Kit (A335225)

Mouse Toxoplasma IgM ELISA Kit

Mouse Toxoplasma IgM ELISA Kit is a 90 minute indirect Enzyme-Linked Immunosorbent Assay (iELISA) designed for the in vitro qualitative determination of mouse Toxoplasma IgM in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.

Indirect (qualitative)

Mouse Toxoplasma IgM ELISA Kit (A335225) is a 1h 30m indirect Enzyme-Linked Immunosorbent Assay (iELISA) designed for the in vitro quantitative determination of mouse Toxoplasma IgM in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. A 96-well microtiter plate has been pre-coated with assay-specific antigen. Standards and samples are added to the wells, where Toxoplasma IgM binds to the immobilized antigen. Following incubation, unbound components are removed by washing and an enzyme-conjugated detection antibody is added to the wells. This detection antibody binds the captured Toxoplasma IgM to form an indirect immune complex. After further incubation and washing, TMB substrate solution is added to visualize the enzyme-mediated reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Toxoplasma IgM present in the sample. The concentration of Toxoplasma IgM is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric Microplate Reader

Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.

1h 30m

Mouse

Specific for Toxoplasma IgM with no reported cross-reactivity with other analogs or variants.

This qualitative ELISA provides only a positive or negative result and does not measure or quantify the concentration of the analyte in the sample.

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.

Information online is representative. Always refer to the Product Manual inside the kit.

This product is for research use only. It is not intended for diagnostic or therapeutic use.