Myelodysplasia Syndrome 1 Cell Based ELISA Kit (A103614)

Myelodysplasia Syndrome 1 Cell Based ELISA Kit

Myelodysplasia Syndrome 1 Cell Based ELISA Kit is a Enzyme-Linked Immunosorbent Assay designed for the indirect in vitro quantitative determination of MECOM levels in adherent and suspension cells.

Cell-based (qualitative)

This Myelodysplasia Syndrome 1 Cell Based ELISA Kit allows for the detection of Myelodysplasia Syndrome 1 and the effects that certain stimulation conditions have on Myelodysplasia Syndrome 1 expression in different cell lines. Qualitative determination of Myelodysplasia Syndrome 1 concentration is achieved by an indirect ELISA format. In essence, the Myelodysplasia Syndrome 1 is captured by Anti-Myelodysplasia Syndrome 1 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this Myelodysplasia Syndrome 1 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

Colorimetric

Adherent and suspension cells.

> 5000 Cells

4 hours 30 minutes

Human

Store at + 4°C. Stable for 3-6 months.

Ecotropic virus integration site 1 protein homolog, EVI-1, Histone-lysine N-methyltransferase MECOM, MDS1, MDS1 and EVI1 complex locus protein, Myelodysplasia syndrome 1 protein, Myelodysplasia syndrome-associated protein 1, PRDM3

Information online is representative. Always refer to the Product Manual inside the kit.