NFkB p100 / p52 Cell Based ELISA Kit (A102828)

NFkB p100 / p52 Cell Based ELISA Kit

Cell-based (qualitative)

This NFkB p100 / p52 Cell Based ELISA Kit allows for the detection of NFkB p100 / p52 and the effects that certain stimulation conditions have on NFkB p100 / p52 expression in different cell lines. Qualitative determination of NFkB p100 / p52 concentration is achieved by an indirect ELISA format. In essence, the NFkB p100 / p52 is captured by Anti-NFkB p100 / p52 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this NFkB p100 / p52 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

Microplate

Colorimetric

> 5000 Cells

4 hours 30 minutes

Human, Mouse, Rat

Store at + 4°C. Stable for 6 months.

CVID10, DNA binding factor KBF2, DNA-binding factor KBF2, H2TF1, Lymphocyte translocation chromosome 10, Lymphocyte translocation chromosome 10 protein, Lyt 10, Lyt10, NF kB2, NFKB2, NFKB2_HUMAN, Nuclear factor NF kappa B p100 subunit, Nuclear factor NF kappa B p52 subunit, Nuclear factor NF-kappa-B p52 subunit, Nuclear factor of kappa light chain gene enhancer in B cells 2, Nuclear factor of kappa light polypeptide gene enhancer in B cells 2, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, Oncogene Lyt 10, Oncogene Lyt-10, p105, p49/p100

Information online is representative. Always refer to the Product Manual inside the kit.