p47 phox (phospho Ser304) Cell Based ELISA Kit (A102783)

p47 phox (phospho Ser304) Cell Based ELISA Kit

p47 phox (phospho Ser304) Cell Based ELISA Kit is a Enzyme-Linked Immunosorbent Assay designed for the indirect in vitro quantitative determination of p47phox levels in adherent and suspension cells.

Cell-based (qualitative)

This p47 phox (phospho Ser304) Cell Based ELISA Kit allows for the detection of p47 phox (phospho Ser304) and the effects that certain stimulation conditions have on p47 phox (phospho Ser304) expression in different cell lines. Qualitative determination of p47 phox (phospho Ser304) concentration is achieved by an indirect ELISA format. In essence, the p47 phox (phospho Ser304) is captured by Anti-p47 phox (phospho Ser304) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this p47 phox (phospho Ser304) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-p47 phox Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.

Colorimetric

Adherent and suspension cells.

> 5000 Cells

4 hours 30 minutes

Human

Store at + 4°C. Stable for 3-6 months.

47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-1, NCF-47K, NCF1, Neutrophil cytosol factor 1, Neutrophil NADPH oxidase factor 1, Nox organizer 2, NOXO2, SH3PXD1A

Information online is representative. Always refer to the Product Manual inside the kit.