PAK1 + 2 + 3 (phospho Ser144 + Ser141 + Ser139) Cell Based ELISA Kit (A102457)

PAK1 + 2 + 3 (phospho Ser144 + Ser141 + Ser139) Cell Based ELISA Kit

PAK1 + 2 + 3 (phospho Ser144 + Ser141 + Ser139) Cell Based ELISA Kit is a Enzyme-Linked Immunosorbent Assay designed for the indirect in vitro quantitative determination of PAK1 + PAK2 + PAK3 levels in adherent and suspension cells.

Cell-based (qualitative)

This PAK1 + 2 + 3 (phospho Ser144 + Ser141 + Ser139) Cell Based ELISA Kit allows for the detection of PAK1 + 2 + 3 (phospho Ser144 + Ser141 + Ser139) and the effects that certain stimulation conditions have on PAK1 + 2 + 3 (phospho Ser144 + Ser141 + Ser139) expression in different cell lines. Qualitative determination of PAK1 + 2 + 3 (phospho Ser144 + Ser141 + Ser139) concentration is achieved by an indirect ELISA format. In essence, the PAK1 + 2 + 3 (phospho Ser144 + Ser141 + Ser139) is captured by Anti-PAK1 + 2 + 3 (phospho Ser144 + Ser141 + Ser139) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this PAK1 + 2 + 3 (phospho Ser144 + Ser141 + Ser139) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-PAK1 + 2 + 3 Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.

Tissue Culture Treated Microplate

Adherent and suspension cells.

> 5000 Cells

4 hours 30 minutes

Human, Mouse, Rat

Store at + 4°C. Stable for 3-6 months.

Alpha PAK, Beta PAK, bPAK, CDKN1A, Gamma PAK, hPAK3, MRX30, MRX47, Oligophrenin 3, OPHN3, P21 (CDKN1A) activated kinase 2, P21 (CDKN1A) activated kinase 3, p21 activated kinase 1, p21 activated kinase 2, p21 activated kinase 3, P21 protein (Cdc42/Rac) activated kinase 1, P21 protein (Cdc42/Rac) activated kinase 2, P21 protein (Cdc42/Rac) activated kinase 3, P21/Cdc42/Rac1 activated kinase 1 (STE20 homolog, yeast), P21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related), P58, P65 PAK, PAK 2, PAK 3, PAK1, PAK2, PAK3, PAK3beta, PAK65, PAKalpha, PAKgamma, S6/H4 kinase, Serine/threonine protein kinase PAK 1, Serine/threonine protein kinase PAK 2, Serine/threonine protein kinase PAK 3, Serine/threonine protein kinase PAK1, Serine/threonine protein kinase PAK2, Serine/threonine protein kinase PAK3, STE20 homolog, yeast

Information online is representative. Always refer to the Product Manual inside the kit.