PDGFR alpha Cell Based ELISA Kit (A103373)

PDGFR alpha Cell Based ELISA Kit

Cell-based (qualitative)

This PDGFR alpha Cell Based ELISA Kit allows for the detection of PDGFR alpha and the effects that certain stimulation conditions have on PDGFR alpha expression in different cell lines. Qualitative determination of PDGFR alpha concentration is achieved by an indirect ELISA format. In essence, the PDGFR alpha is captured by Anti-PDGFR alpha Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this PDGFR alpha Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

Microplate

Adherent cells and suspension cells.

> 5000 Cells

4 hours 30 minutes

Human, Mouse

Store at + 4°C. Stable for 6 months.

Alpha platelet-derived growth factor receptor, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, CD140a, CD140a antigen, PDGF-R-alpha, PDGFR-2, PDGFR-alpha, PDGFR2, PDGFRA, Platelet-derived growth factor alpha receptor, Platelet-derived growth factor receptor 2, Platelet-derived growth factor receptor alpha, RHEPDGFRA

Information online is representative. Always refer to the Product Manual inside the kit.