PKD1 Cell Based ELISA Kit (A103255)

PKD1 Cell Based ELISA Kit

Cell-based (qualitative)

This PKD1 Cell Based ELISA Kit allows for the detection of PKD1 and the effects that certain stimulation conditions have on PKD1 expression in different cell lines. Qualitative determination of PKD1 concentration is achieved by an indirect ELISA format. In essence, the PKD1 is captured by Anti-PKD1 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this PKD1 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

Microplate

Colorimetric

> 5000 Cells

4 hours 30 minutes

Human, Mouse, Rat

Store at + 4°C. Stable for 6 months.

Autosomal dominant polycystic kidney disease 1 protein, Autosomal dominant polycystic kidney disease protein 1, nPKC-D1, nPKC-mu, OTTHUMP00000208669, OTTHUMP00000208670, PBP, Pc-1, PKD, PKD1_HUMAN, Polycystic Kidney Disease 1, polycystic kidney disease-associated protein, Polycystin 1, Polycystin 1 Precursor, Polycystin-1, Protein kinase C mu type, Protein kinase D, Serine/threonine-protein kinase D1, transient receptor potential cation channel, subfamily P, member 1, TRPP1

Information online is representative. Always refer to the Product Manual inside the kit.