Porcine Anti-SARS-CoV2 (Delta B.1.617.2) Antibody ELISA Kit (A350900)

Porcine Anti-SARS-CoV2 (Delta B.1.617.2) Antibody ELISA Kit

Porcine Anti-SARS-CoV2 (Delta B.1.617.2) Antibody ELISA Kit is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of porcine Anti-SARS-CoV2 (Delta B.1.617.2) Antibody in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.

Sandwich (quantitative)

Porcine Anti-SARS-CoV2 (Delta B.1.617.2) Antibody ELISA Kit (A350900) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of porcine Anti-SARS-CoV2 (Delta B.1.617.2) Antibody in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. A 96-well microtiter plate has been pre-coated with assay-specific antigen. Standards and samples are added to the wells, where Anti-SARS-CoV2 (Delta B.1.617.2) Antibody binds to the immobilized antigen. Following incubation, unbound components are removed by washing and a biotinylated antigen detection reagent is added to the wells. This reagent binds the captured Anti-SARS-CoV2 (Delta B.1.617.2) Antibody to form an antigen-antibody-antigen sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Anti-SARS-CoV2 (Delta B.1.617.2) Antibody captured in each well. The concentration of Anti-SARS-CoV2 (Delta B.1.617.2) Antibody is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric Detection (OD450)

Colorimetric Microplate Reader

Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.

3.125-200 ng/mL

4 hours

Porcine

Demonstrates specificity for anti-SARS-CoV2 (Delta B.1.617.2) antibodies with no reported cross-reactivity to related analogs.

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.

Information online is representative. Always refer to the Product Manual inside the kit.

This product is for research use only. It is not intended for diagnostic or therapeutic use.