Rat Adiponectin ELISA Kit (A327156)

Rat Adiponectin ELISA Kit

Rat Adiponectin ELISA Kit is a 90 minute sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the rapid in vitro quantitative determination of rat Adiponectin in serum and plasma.

Sandwich (quantitative)

Rat Adiponectin ELISA Kit (A327156) employs a rapid sandwich Enzyme-Linked Immunosorbent Assay (ELISA) technique for the quantitative measurement of rat Adiponectin in serum and plasma. An antibody specific for Adiponectin has been pre-coated onto a 96-well microtiter plate. Standards and test samples are added to the wells together with HRP-conjugated detection antibody working solution. During incubation, Adiponectin present in the sample binds to the immobilized capture antibody and forms a sandwich immune complex with the HRP-conjugated detection antibody. Following incubation, unbound components are removed by washing. TMB substrate solution is then added to visualize the HRP enzymatic reaction, producing a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Adiponectin captured in each well. The concentration of Adiponectin is determined by measuring the O.D. absorbance at 450 nm using a microplate reader and comparing the values to the standard curve.

Pre-Coated 96 Well Microplate, Reference Standard, HRP Conjugate, Concentrated HRP Conjugate, Reference Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer (25X), TMB Substrate, Stop Solution, Plate Sealers, Product Manual.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric Detection (OD450)

Serum and plasma.

6.43 ng/mL

15.63-1000 ng/mL

1h 30m

Rat

Specific for Adiponectin with no reported cross-reactivity with other analogs or variants.

Information online is representative. Always refer to the Product Manual inside the kit.

Shipped at +4°C. Store kit components at +4°C as instructed in the Protocol Manual for up to 6 months. Do not use past expiration date!

30 kDa adipocyte complement-related protein, ACDC, ACRP30, Adipocyte complement-related 30 kDa protein, Adipocyte, C1q and collagen domain-containing protein, ADIPOQ, Adipose most abundant gene transcript 1 protein, apM-1, APM1, GBP28, Gelatin-binding protein

Information online is representative. Always refer to the Product Manual inside the kit.