Rat Estrogen Related Receptor alpha ELISA Kit (A8135)

Rat Estrogen Related Receptor alpha ELISA Kit

Rat Estrogen Related Receptor alpha ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat Estrogen Related Receptor alpha in tissue homogenates, cell lysates or other biological fluids.

Sandwich (quantitative)

Rat Estrogen Related Receptor alpha ELISA Kit (A8135) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat Estrogen Related Receptor alpha in tissue homogenates, cell lysates or other biological fluids. An antibody specific for Estrogen Related Receptor alpha has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Estrogen Related Receptor alpha present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Estrogen Related Receptor alpha Antibody, which binds the captured Estrogen Related Receptor alpha present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Estrogen Related Receptor alpha captured in each well. The concentration of Estrogen Related Receptor alpha can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colourmetric

Tissue homogenates, cell lysates or other biological fluids.

0.053 ng/ml

0.156-10 ng/ml

4h 30m

Rat

Intra-assay: CV < 10%, Inter-assay: CV < 12%

Store kit components at +4°C or -20°C as instructed. Do not use past expiration date!

Err 1, ERR a, ERR alpha, ERR-alpha, Err1, ERR1 protein, ERR1_HUMAN, ERRa, ERRalpha, ESRL 1, ESRL1, ESRR A, Esrra, Estrogen receptor like 1, Estrogen receptor related 1, estrogen receptor related receptor alpha, Estrogen receptor-like 1, Estrogen-related receptor alpha, Estrra, hERR1, NR3B1, Nuclear receptor subfamily 3 group B member 1, Steroid hormone receptor ERR1

Information online is representative. Always refer to the Product Manual inside the kit.