Rat ICAM1 ELISA Kit (A1625)

Rat ICAM1 ELISA Kit

Rat ICAM1 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat ICAM1 in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Rat sICAM-1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of rat sICAM-1 in the samples. A monoclonal antibody specific for rat sICAM-1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for sICAM-1, and sICAM-1 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of sICAM-1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

0.48 pg/ml

62.50 - 4000 pg/ml

This kit can assay both native and recombinant rat sICAM-1.

Rat

This ELISA kit has shown no significant cross-reactivity.

83% (Average Recovery from Serum Samples).

Store at +4°C. Do not use past expiration date!

ICAM-1, Intercellular adhesion molecule 1, Major group rhinovirus receptor

Information online is representative. Always refer to the Product Manual inside the kit.