Rat IL-17A ELISA Kit (A1620)

Rat IL-17A ELISA Kit

Rat IL-17A ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat IL17A in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Rat IL-17A ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of rat IL-17A in the samples. A monoclonal antibody specific for rat IL-17A has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for IL-17A, and IL-17A present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-17A bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

0.62 pg/ml

3.13 - 200 pg/ml

This kit can assay both native and recombinant rat IL-17A.

Rat

This ELISA kit has shown no significant cross-reactivity.

103% (Average Recovery from Serum Samples).

Store at +4°C. Do not use past expiration date!

CTLA-8, CTLA8, Cytotoxic T-lymphocyte-associated antigen 8, IL-17, IL17, Interleukin-17A

Information online is representative. Always refer to the Product Manual inside the kit.