Rat TIM 1 ELISA Kit (A351883)

Rat TIM 1 ELISA Kit

Rat TIM 1 ELISA Kit is a 90 minute sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the rapid in vitro quantitative determination of rat TIM 1 in serum, plasma, and other biological fluids.

Sandwich (quantitative)

Rat TIM 1 ELISA Kit (A351883) employs a rapid sandwich Enzyme-Linked Immunosorbent Assay (ELISA) technique for the quantitative measurement of rat TIM 1 in serum, plasma, and other biological fluids. An antibody specific for TIM 1 has been pre-coated onto a 96-well microtiter plate. Standards and test samples are added to the wells together with HRP-conjugated detection antibody working solution. During incubation, TIM 1 present in the sample binds to the immobilized capture antibody and forms a sandwich immune complex with the HRP-conjugated detection antibody. Following incubation, unbound components are removed by washing. TMB substrate solution is then added to visualize the HRP enzymatic reaction, producing a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of TIM 1 captured in each well. The concentration of TIM 1 is determined by measuring the O.D. absorbance at 450 nm using a microplate reader and comparing the values to the standard curve.

Pre-Coated 96 Well Microplate, Reference Standard, HRP Conjugate, Concentrated HRP Conjugate, Reference Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer (25X), TMB Substrate, Stop Solution, Plate Sealers, Product Manual.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric Detection (OD450)

Serum, plasma, and other biological fluids.

0.19 ng/mL

0.31-20 ng/mL

1h 30m

Rat

Specific for TIM 1 with no reported cross-reactivity with other analogs or variants.

Information online is representative. Always refer to the Product Manual inside the kit.

Store kit components at +4°C or -20°C as instructed. Do not use past expiration date!

CD365, HAVcr-1, HAVCR1, Hepatitis A virus cellular receptor 1, Kidney injury molecule 1, KIM-1, KIM1, T-cell immunoglobulin and mucin domain-containing protein 1, T-cell immunoglobulin mucin receptor 1, T-cell membrane protein 1, TIM, TIM-1, TIM1, TIMD-1, TIMD1

Information online is representative. Always refer to the Product Manual inside the kit.