Rat TNF-a ELISA Kit (A1627)

Rat TNF-a ELISA Kit

Rat TNF-a ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat TNF-alpha in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Rat TNF-a ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of rat TNF-a in the samples. A monoclonal antibody specific for rat TNF-a has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for TNF-a, and TNF-a present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of TNF-a bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

7.14 pg/ml

78.13 - 5000 pg/ml

This kit can assay both native and recombinant rat TNF-a.

Rat

This ELISA kit has shown no significant cross-reactivity.

96% (Average Recovery from Serum Samples).

Store at +4°C. Do not use past expiration date!

Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor, Tumor necrosis factor ligand superfamily member 2

Information online is representative. Always refer to the Product Manual inside the kit.