S1P Receptor EDG1 Cell Based ELISA Kit (A102821)

S1P Receptor EDG1 Cell Based ELISA Kit

S1P Receptor EDG1 Cell Based ELISA Kit is a Enzyme-Linked Immunosorbent Assay designed for the indirect in vitro quantitative determination of S1P1 levels in adherent and suspension cells.

Cell-based (qualitative)

This S1P Receptor EDG1 Cell Based ELISA Kit allows for the detection of S1P Receptor EDG1 and the effects that certain stimulation conditions have on S1P Receptor EDG1 expression in different cell lines. Qualitative determination of S1P Receptor EDG1 concentration is achieved by an indirect ELISA format. In essence, the S1P Receptor EDG1 is captured by Anti-S1P Receptor EDG1 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this S1P Receptor EDG1 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

Colorimetric

Adherent and suspension cells.

> 5000 Cells

4 hours 30 minutes

Human, Mouse, Rat

Store at + 4°C. Stable for 3-6 months.

CD363, CHEDG1, EDG1, Endothelial differentiation G-protein coupled receptor 1, S1P receptor 1, S1P receptor Edg-1, S1PR1, Sphingosine 1-phosphate receptor 1, Sphingosine 1-phosphate receptor Edg-1

Information online is representative. Always refer to the Product Manual inside the kit.