SEK1 (phospho Thr261) Cell Based ELISA Kit (A102258)

SEK1 (phospho Thr261) Cell Based ELISA Kit

Cell-based (qualitative)

This SEK1 (phospho Thr261) Cell Based ELISA Kit allows for the detection of SEK1 (phospho Thr261) and the effects that certain stimulation conditions have on SEK1 (phospho Thr261) expression in different cell lines. Qualitative determination of SEK1 (phospho Thr261) concentration is achieved by an indirect ELISA format. In essence, the SEK1 (phospho Thr261) is captured by Anti-SEK1 (phospho Thr261) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this SEK1 (phospho Thr261) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-SEK1 Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.

Microplate

Adherent cells and suspension cells.

> 5000 Cells

4 hours 30 minutes

Human, Mouse, Rat

Store at + 4°C. Stable for 6 months.

c-Jun N-terminal kinase kinase 1, Dual specificity mitogen-activated protein kinase kinase 4, JNK-activating kinase 1, JNKK, JNKK1, MAP kinase kinase 4, MAP2K4, MAPK/ERK kinase 4, MAPKK 4, MEK 4, MEK4, PRKMK4, SAPK kinase 1, SAPK/ERK kinase 1, SAPKK-1, SAPKK1, SERK1, SKK1, Stress-activated protein kinase kinase 1

Information online is representative. Always refer to the Product Manual inside the kit.