Sheep Cortisol ELISA Kit is a 90 minute competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the rapid in vitro quantitative determination of sheep Cortisol in serum and plasma.
Assay Type
Competitive (quantitative)
Principle of Assay
Sheep Cortisol ELISA Kit (A327162) employs a rapid competitive Enzyme-Linked Immunosorbent Assay (ELISA) technique for the quantitative measurement of sheep Cortisol in serum and plasma. Antigen specific for Cortisol has been pre-coated onto a 96-well microtiter plate. Standards and test samples are added to the wells together with HRP-conjugated detection reagent working solution. During incubation, Cortisol present in the sample competes with the immobilized antigen for binding to the HRP-conjugated detection reagent. Following incubation, unbound components are removed by washing. TMB substrate solution is then added to visualize the HRP enzymatic reaction, producing a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is inversely proportional to the concentration of Cortisol present in the sample. The concentration of Cortisol is determined by measuring the O.D. absorbance at 450 nm using a microplate reader and comparing the values to the standard curve.
Platform
Pre-coated Microplate (12 x 8 well strips)
Detection Type
Colorimetric Detection (OD450)
Instrument
Colorimetric Microplate Reader
Sample Type
Serum and plasma.
Sensitivity
1.13 ng/mL
Product Range
3.13-200 ng/mL
Assay Time
1h 30m
Reactivity
Sheep
Cross Reactivity
Specific for Cortisol with no reported cross-reactivity with other analogs or variants.
General Notes
Information online is representative. Always refer to the Product Manual inside the kit.
Storage
Shipped at +4°C. Store kit components at +4°C as instructed in the Protocol Manual for up to 6 months. Do not use past expiration date!
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Linearity assessment of the Sheep Cortisol ELISA Kit (A327162) across serial sample dilutions. Linearity was evaluated using Serum and EDTA Plasma samples serially diluted from 1:2 to 1:16. Recovery (%) was calculated relative to the expected Sheep Cortisol concentration at each dilution. Data are presented as mean recovery values from 8 replicates (n = 8).
Quick workflow summary for 90 Minute Competitive ELISA Kits. Standards or samples (50 µL per well) are added to antigen pre-coated microplate wells together with HRP-conjugated detection reagent working solution. During incubation, analytes present in the sample competitively inhibit binding of the HRP-conjugated reagent to the immobilized antigen coating. Following incubation and wash steps to remove unbound components, TMB substrate is added for color development. The enzymatic reaction is terminated using stop solution prior to optical density measurement at 450 nm. In this competitive ELISA format, signal intensity is inversely proportional to analyte concentration in the sample.
Streamlined rapid competitive ELISA workflow for fast and sensitive analyte quantification. This rapid 90-minute competitive ELISA workflow enables sensitive and reliable quantitative detection of target analytes in biological samples using a simplified incubation and detection protocol. Target analytes present in the sample compete with immobilized antigen for binding to specific detection antibodies during incubation. Following substrate development, colorimetric signal intensity is measured and is inversely proportional to analyte concentration within the sample. This optimized workflow supports high-performance ELISA analysis with reduced assay time and efficient sample processing.
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