SLP-76 (phospho Tyr128) Cell Based ELISA Kit is a Enzyme-Linked Immunosorbent Assay designed for the indirect in vitro quantitative determination of SLP76 levels in adherent and suspension cells.
Assay Type
Cell-based (qualitative)
Principle of Assay
This SLP-76 (phospho Tyr128) Cell Based ELISA Kit allows for the detection of SLP-76 (phospho Tyr128) and the effects that certain stimulation conditions have on SLP-76 (phospho Tyr128) expression in different cell lines. Qualitative determination of SLP-76 (phospho Tyr128) concentration is achieved by an indirect ELISA format. In essence, SLP-76 (phospho Tyr128) is captured by Anti-SLP-76 (phospho Tyr128) Antibodies while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the dye conjugated to the secondary antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. Anti-SLP-76 Antibody is also included for normalization purposes. The RFU values obtained for the non-phosphorylated target can be used to normalize the RFU value for the phosphorylated target.
Platform
Tissue Culture Treated Microplate
Detection Type
Fluorometric
Sample Type
Adherent and suspension cells.
Product Range
> 5000 Cells
Reactivity
Human, Mouse, Rat
Storage
Store at + 4°C. Stable for 3-6 months.
Synonyms
LCP2, Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76 kDa, SLP-76 tyrosine phosphoprotein
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - SLP-76 (phospho Tyr128) Cell Based ELISA Kit (A103778)
Western blot analysis of lysates from K562 cells treated with Na3VO4 0.3nM 40', using Anti-SLP-76 (phospho Tyr128) Antibody. The lane on the right is blocked with the phospho peptide.
Western Blot - SLP-76 (phospho Tyr128) Cell Based ELISA Kit (A103778)
The mouse monoclonal antibody to GAPDH is used as a positive control in SLP-76 (phospho Tyr128) Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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Proteins predicted to interact with SLP76
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
