Recombinant Staphylococcus aureus Protein A ELISA Kit (A334807)

Staphylococcus aureus Protein A ELISA Kit

Staphylococcus aureus Protein A ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of Staphylococcus aureus Protein A in cell culture supernatant.

Sandwich (quantitative)

Staphylococcus aureus Protein A ELISA Kit (A334807) employs the sandwich enzyme immunoassay technique for the quantitative measurement of Staphylococcus aureus Protein A in cell culture supernatant. An antibody specific for Staphylococcus aureus Protein A has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Staphylococcus aureus Protein A present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Staphylococcus aureus Protein A Antibody, which binds the captured Staphylococcus aureus Protein A present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Staphylococcus aureus Protein A captured in each well. The concentration of Staphylococcus aureus Protein A can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

This ELISA Kit contains 10X 600 µL Capture Antibodies, 10X 600 µL Detector Antibodies, 2 Lyophilized Recombinant Protein Vials, 50 mL Assay Diluent #8, 4X 10 mL PTL2 Buffer, 4X 10 mL PTA3 Buffer, 4X 10 mL PTBH2 Buffer, 10X 20 mL Wash Buffer, 12 mL TMB Solution, 12 mL Stop Solution, Pre-Coated 96-Well H-Microplate (12 x 8 well strips), and 2 Plate Seals.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric

Cell culture supernatant.

8.910 pg/ml

39.06 pg/ml - 2500 pg/ml

1 hour

Rabbit IgG: 89%; Human IgG: 110%; Mouse IgG: 113%

Intra-assay: 4.7%, Inter-assay: 6.3%

The immunogens for the antibodies in this kit are recombinant Staphylococcus aureus Protein A.

Recombinant protein is supplied lyophilized; antibodies and buffer are supplied in liquid form.

Antibodies are supplied in Phosphate Buffered Saline with Tris-HCl.

Shipped at +4 °C. For short-term storage, keep at +4 °C. Shelf life is 12 months from the date of receipt or reconstitution.

Information online is representative. Always refer to the Product Manual inside the kit.

This product is for research use only. It is not intended for diagnostic or therapeutic use.