Staphylococcus aureus Protein A ELISA Kit (A334807)

Staphylococcus aureus Protein A ELISA Kit

Staphylococcus aureus Protein A ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of Staphylococcus aureus Protein A in cell culture supernatant.

Sandwich (quantitative)

Staphylococcus aureus Protein A ELISA Kit (A334807) employs a one-step sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of Staphylococcus aureus Protein A in cell culture supernatant. A 96-well microtiter plate is provided pre-coated with an antibody specific for Staphylococcus aureus Protein A. Standards and test samples are added to the wells, allowing Staphylococcus aureus Protein A to bind to the immobilized capture antibody. A prepared antibody mixture is added and incubated to form antibody–antigen complexes. After incubation, unbound material is removed by washing, and TMB substrate is added to develop colour through an HRP-catalysed reaction. The reaction is stopped with acidic stop solution, producing a yellow endpoint signal. The intensity of the colour is proportional to the amount of Staphylococcus aureus Protein A captured in each well. The concentration of Staphylococcus aureus Protein A is determined by measuring absorbance at 450 nm and interpolating from the standard curve.

This ELISA Kit contains 10X 600 µL Capture Antibodies, 10X 600 µL Detector Antibodies, 2 Lyophilized Recombinant Protein Vials, 50 mL Assay Diluent #8, 4X 10 mL PTL2 Buffer, 4X 10 mL PTA3 Buffer, 4X 10 mL PTBH2 Buffer, 10X 20 mL Wash Buffer, 12 mL TMB Solution, 12 mL Stop Solution, Pre-Coated 96-Well H-Microplate (12 x 8 well strips), and 2 Plate Seals.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric

Cell culture supernatant.

8.910 pg/ml

39.06 pg/ml - 2500 pg/ml

1 hour

Rabbit IgG: 89%; Human IgG: 110%; Mouse IgG: 113%

Intra-assay: 4.7%, Inter-assay: 6.3%

The immunogens for the antibodies in this kit are recombinant Staphylococcus aureus Protein A.

Recombinant protein is supplied lyophilized; antibodies and buffer are supplied in liquid form.

Antibodies are supplied in Phosphate Buffered Saline with Tris-HCl.

This ELISA kit uses recombinant rabbit monoclonal antibodies.

Shipped at +4 °C. For short-term storage, keep at +4 °C. Shelf life is 12 months from the date of receipt or reconstitution.

Information online is representative. Always refer to the Product Manual inside the kit.

This product is for research use only. It is not intended for diagnostic or therapeutic use.