Principle of Assay
T2 Toxin ELISA Test Kit (A327255) employs the competitive enzyme immunoassay technique for the quantitative measurement of T2 Toxin in peanuts, corn, oats, soybeans and feed. The 96-well microtiter plate has been pre-coated with T2 Toxin. During the incubation, T2 Toxin present in the samples or standards competes with the fixed amount of immobilized T2 Toxin for binding sites of the Anti-T2 Toxin Antibody, a HRP-Conjugated Secondary Antibody is added to the wells at the same time. The more T2 Toxin present in a sample or standard, the less Anti-T2 Toxin Antibody that binds to the plate. Following incubation, unbound HRP-Conjugated Secondary Antibody is removed by washing. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of T2 Toxin present in each sample or standard. The concentration of T2 Toxin can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
