TBC1D3 Cell Based ELISA Kit (A103663)

TBC1D3 Cell Based ELISA Kit

Cell-based (qualitative)

This TBC1D3 Cell Based ELISA Kit allows for the detection of TBC1D3 and the effects that certain stimulation conditions have on TBC1D3 expression in different cell lines. Qualitative determination of TBC1D3 concentration is achieved by an indirect ELISA format. In essence, the TBC1D3 is captured by Anti-TBC1D3 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this TBC1D3 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

Microplate

Adherent cells and suspension cells.

> 5000 Cells

4 hours 30 minutes

Human

Store at + 4°C. Stable for 6 months.

PRC17, Prostate cancer gene 17 protein, Protein TRE17-alpha, Rab GTPase-activating protein PRC17, TBC1 domain family member 3, TBC1 domain family member 3F, TBC1D3A, TBC1D3E, TBC1D3F

Information online is representative. Always refer to the Product Manual inside the kit.