TISB (phospho Ser92) Cell Based ELISA Kit (A102433)

TISB (phospho Ser92) Cell Based ELISA Kit

TISB (phospho Ser92) Cell Based ELISA Kit is a Enzyme-Linked Immunosorbent Assay designed for the indirect in vitro quantitative determination of ZFP36L1 levels in adherent and suspension cells.

Cell-based (qualitative)

This TISB (phospho Ser92) Cell Based ELISA Kit allows for the detection of TISB (phospho Ser92) and the effects that certain stimulation conditions have on TISB (phospho Ser92) expression in different cell lines. Qualitative determination of TISB (phospho Ser92) concentration is achieved by an indirect ELISA format. In essence, the TISB (phospho Ser92) is captured by Anti-TISB (phospho Ser92) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this TISB (phospho Ser92) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-TISB Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.

Colorimetric

Adherent and suspension cells.

> 5000 Cells

4 hours 30 minutes

Human, Mouse, Rat

Store at + 4°C. Stable for 3-6 months.

BERG36, BRF1, Butyrate response factor 1, EGF-response factor 1, ERF-1, ERF1, mRNA decay activator protein ZFP36L1, RNF162B, TIS11B, TPA-induced sequence 11b, ZFP36-like 1, Zinc finger protein 36, C3H1 type-like 1

Information online is representative. Always refer to the Product Manual inside the kit.