TISD Cell Based ELISA Kit (A103541)

TISD Cell Based ELISA Kit

TISD Cell Based ELISA Kit is a Enzyme-Linked Immunosorbent Assay designed for the indirect in vitro quantitative determination of TIS11D levels in adherent and suspension cells.

Cell-based (qualitative)

This TISD Cell Based ELISA Kit allows for the detection of TISD and the effects that certain stimulation conditions have on TISD expression in different cell lines. Qualitative determination of TISD concentration is achieved by an indirect ELISA format. In essence, the TISD is captured by Anti-TISD Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this TISD Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

Tissue Culture Treated Microplate

Adherent and suspension cells.

> 5000 Cells

4 hours 30 minutes

Human, Mouse

Store at + 4°C. Stable for 3-6 months.

Butyrate response factor 2, EGF-response factor 2, ERF-2, ERF2, mRNA decay activator protein ZFP36L2, RNF162C, TPA-induced sequence 11d, ZFP36-like 2, ZFP36L2, Zinc finger protein 36, C3H1 type-like 2

Information online is representative. Always refer to the Product Manual inside the kit.